Technology based on expression microarrays, proteomic microarrays and tissue microarrays in mammalian oncogenesis research

Autor: 
BARTOSZ KEMPISTY, PIOTR ZAWIERUCHA, HANNA PIOTROWSKA, MICHAŁ NOWICKI
Journal: 
Medycyna Wet
Rok: 
2011
Volume: 
67 (11)
Strona: 
720
Abstract: 

Technological progress in the past few years has made it possible to apply techniques based on microarrays
in the analyses of cancerogenesis. These techniques can be divided into several groups according to the scale of
research: (1) gene expression analysis, (2) protein analysis, (3) tissue research. The gene expression analysis
makes it possible to compare the levels of gene activity in cancer tissue with those in reference tissue, and to
evaluate the progression of cancer and its reaction to treatment. The analysis of proteins provides information
on their functional and structural characteristics, and throws light on the protein-ligand interaction and the
relations between the presence or absence of certain proteins in specific physiological states. Tissue microarrays
are applied to analyze cancer markers and to identify DNA, RNA and proteins.
This article presents selected aspects of microarray research and discusses the molecular aspects of
cancerogenesis in dogs, referring to several types of genes associated with cancers.